Chinese Physics C > 2005, Vol. 29 > Issue(S1): 4-7

Small Angle X-ray Scattering Study of the Influence of Temperature on the Structure of DNA/Lipid Complexes

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Abstract：
We report on the synchrotron small-angle X-ray scattering (SAXS) study of self-assembled complex formed by mixing short-chain DNAs with cationic phospholipids in water. The DNA/lipid complex is a sandwiched structure, consisting of alternate layers of DNAs and lipid bilayers with a periodicity of d _D/L. The DNAs at the interfaces are one-dimensionally ordered with a periodicity of d _DNA. When the temperature of the system is increased, the lipid bilayers become soft so that the DNAs are trapped more deeply inside the lipid bilayers. This explains why the measured d _D/Ldecreases insignificantly with the increase of temperature.On the other hand, the value of d _DNAkeeps constant because of the stiffness of the DNAs.

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HU Shu-Xin, LI Da-Peng, MAI Zhen-Hong and LI Ming. Small Angle X-ray Scattering Study of the Influence of Temperature on the Structure of DNA/Lipid Complexes[J]. Chinese Physics C, 2005, 29(S1): 4-7.

HU Shu-Xin, LI Da-Peng, MAI Zhen-Hong and LI Ming. Small Angle X-ray Scattering Study of the Influence of Temperature on the Structure of DNA/Lipid Complexes[J]. Chinese Physics C, 2005, 29(S1): 4-7. shu

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Received: 2005-10-28
Revised: 1900-01-01

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通讯作者:陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

Small Angle X-ray Scattering Study of the Influence of Temperature on the Structure of DNA/Lipid Complexes

Corresponding author:LI Ming,

Received Date:2005-10-28

Accepted Date:1900-01-01

Available Online:2005-01-02

Abstract:We report on the synchrotron small-angle X-ray scattering (SAXS) study of self-assembled complex formed by mixing short-chain DNAs with cationic phospholipids in water. The DNA/lipid complex is a sandwiched structure, consisting of alternate layers of DNAs and lipid bilayers with a periodicity of d_D/L. The DNAs at the interfaces are one-dimensionally ordered with a periodicity of d_DNA. When the temperature of the system is increased, the lipid bilayers become soft so that the DNAs are trapped more deeply inside the lipid bilayers. This explains why the measured d_D/Ldecreases insignificantly with the increase of temperature.On the other hand, the value of d_DNAkeeps constant because of the stiffness of the DNAs.

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Small Angle X-ray Scattering Study of the Influence of Temperature on the Structure of DNA/Lipid Complexes

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